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Products> Research
GAYFC-HRP: Affinity-Purified Goat Anti-IgY Fc Antibody, HRP Conjugate
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place in shopping cart Price: enquiry € / 0.2 mg
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| Presentation: | 50 mM HEPES ph7.0, 0.1 M Sodium Chloride, 0.2% BSA, 0,01% Thimerosal |
| General Information: | Goats were immunized with highly purified chicken IgY Fc fragment isolated from normal chicken yolk IgY. The resulting anti-IgY Fc antibody was passed over a Fab immunoabsorbant and the anti-Fc Ab were immunoaffinity purified off a chicken IgY immunoabsorbant column and conjugated with horse radish peroxidase (HRP). |
| Applications: | Western Blot, IHC |
| Staining procedure: | The affinity purified antibodies react only with chicken IgY and not IgM or IgA immunoglobulins by immunodiffusion and IEP techniques. In a sandwich ELISA against 1 mg/mL chicken IgY, conjugated antibody diluted to 1:100,000 produced an O.D. = 1.0 in a 15 minute reaction with TMB substrate. Recommended Western Blot dilution: 1:2,000 - 1:4,000 when used for colorimetric assay, and 1:10,000 or up for ECL detection system.Optimal conjugate working dilutions should be determined for your particular assay conditions. |
| Storage: | 4°C or -20°C, avoid frequent freeze-thaw |
Notes:
This product is produced by GENWAY Laboratories (San Diego, CA) and licensed to BIOLOGO (Germany). The product is intended for in vitro research use only, not for diagnostic or therapeutic applications.
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BioLogo | Steindamm 1 G-H | 24119 Kronshagen | Germany | Phone +49 (0)431.5842 801 | Fax +49 (0)431.5842 803 | info@biologo.de
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