Research - DEF03-A: Beta-Defensin-3 (HBD-3/MBD-3), affinity purified

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DEF03-A: Beta-Defensin-3 (HBD-3/MBD-3), affinity purified

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Price: 400 € / 100 µg

Polyclonal Antibodies

Origin:	Rabbit

Isotype:	IgG

Clone:	polyclonal

Format:	lyophilisate

Quantity:	100 µg

Working concentration:	1-10 µg/ml Western Blot, 0.5-1 µg/ml ELISA, IHC 2-10 µg/ml (not tested)

Presentation:	Peptide Sepharose purified IgG in PBS, BSA, lyophilized without preservative. Reconstitute with 0.1 ml sterile water to get an antibody concentration of 1 mg/ml. Dilution buffers are depending on the type of application.

Immunogen:	Mixture of synthetic peptides 15 aa near C-terminus of the mature HBD-3 (LPKEEQIGKCSTRGR) and 13 aa of N-terminal MBD-3, coupled to KLH for immunisation of rabbits

Specificity:	ß-defensin-3 of human and mouse origin, N-terminal fragments of HBD-3 and MBD-3, no cross-reactivity with other known ß-defensins or alpha-defensins

Species Specificity:	Human, mouse

General Information:	Human defensins are Cys-rich antimicrobial peptides which specifically react against Gram-negative bacteria. Originally defensins were isolated from hemolysates, but they can also be found on the cell surface of epithelia of the urogenital tract, lung, and trachea. In leukocytes defensins are stored in granules being released into phagolysosomes for the destruction of incorporated micro-organisms. Amino acid sequences of alpha-defensins 1-4 (HNP1-4) and beta-Defensins 1-4 (HBD1-4) are known. Mature Human Beta-Defensin-3 (HBD-3) shares app. 22% aa sequence homology with HBD-1.

Special properties:	The peptide sequences of HBD-3/MBD-3 used for immunisation contain very little consensus aa with any of the other human or mouse defensins.

Positive control:	Skin and airway epithelia

Applications:	ELISA, RIA, IHC not established

Secondary reagents:	We recommend the VECTASTAIN Elite ABC Kit Rabbit IgG (Ref. PK-6101) or BioPrime Universal Staining Kits (Ref. AE005 and DA005).

Storage:	-20'°C'

References:
1. Harder J., Bartels J., Christophers E., and J.-M. Schröder (1997) Antibacterial peptide specific for Gram-negative Bacteria / also effective for Candida albicans. Nature 387; 861ff

2. Bals R., Wang X., Meegalla R. L., Wattler S., et al. (1999) Mouse ß-Defensin 3 is an inducible antimicrobial peptide expressed in the epithelia of multiple organs. Infection and Immunity 67(7); 3542-3547.

3. Jia H.P., Schutte B.C., Schudy A., Linzmeier R., et al. (2001) Discovery of new human beta-defensins using a genomics-based approach. Gene 263(1-2);211-218.

4. Garcia J.R., Jaumann F., Schulz S., Krause A., et al. (2001) Identification of a novel multifunctional beta-defensin (human beta-defensin 3) with specific antimicrobial activity. Its interaction with plasma membranes of Xenopus oocytes and the induction of macrophage chemoattraction. Cell Tissue Res. 306(2); 257-264.

5. Harder J., Bartels J., Christophers E., and J.-M. Schröder (2001) Isolation and characterization of human beta-defensin-3, a novel human inducible peptide antibiotic. J. Biol. Chem. 276(8); 5707-5713.

Notes:
BIOLOGO antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.