ECM - LA24811: Laminin, human

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LA24811: Laminin, human

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Price: 425 € / 0.5 ml

Polyclonal Antibodies

Origin:	Rabbit

Isotype:	Ig

Clone:	polyclonal

Format:	purified, lyophilized

Quantity:	0.5 ml

Reconstitution:	Reconstitute with 0.5 ml distilled water, store aliquots at -20°C, for further dilution use appropriate antibody diluent e.g. Art. No. PU002

Concentration:	app. 1 mg/ml

Working concentration:	IFA 1:40, IHC(P) 1:200 - 1:600

Presentation:	0.5 ml lyophilized IgG-fraction from ion- exchange chromatography

Immunogen:	Purified laminin P1 fragment from human placenta

Specificity:	Human laminin fragment P1 100%, human collagen type I and IV <0.1%, human fibronectin <0.1% (RIA at 1:1000 dilution)

Species Specificity:	Human

General Information:	Laminins are glycoproteins of the extracellular matrix, especially of the basal membranes. They are multi-functional molecules which play an important role during development and differentiation of organs and for cell movements. These properties rely on the interaction of laminins with receptors on cell surfaces like integrins, collagen IV, proteoglycans. Laminins consist of 2 heavy chains (A and M, 300-400 kDa) and 1 light chain (B1, B2 or S, 200 - 220 kDa).

Special properties:	The antibody against Laminin primarily labels basal membranes (especially s. c. lamina lucida) and may be used to detect disturbances in the basal membrane during tumour invasion. Laminin is also found in extracellular matrix not related to basal membranes, e.g. in the proximity of neurons especially during development, regeneration and tumour development.

Positive control:	Human skin

Pre-treatment:	After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg e.g. Art. No. HYA02-50) in TBS 15 min at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.

Applications:	IFA, IHC(P), ELISA, RIA

Incubation time:	IHC(P) 60 min at RT or 2-8°C over night

Secondary reagents:	Anti-rabbit IgG-conjugates, e.g. anti-rabbit IgG:Biotin (Art. No. BA-1000)

Storage:	2 - 8°C or aliquots at -20°C.

References:
See list of references in BIOLOGO ECM brochure.

Notes:
BIOLOGO antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.