ECM - FI24931: Fibronectin, chicken

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FI24931: Fibronectin, chicken

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Price: 425 € / 0.5 ml

Polyclonal Antibodies

Origin:	Rabbit

Isotype:	Ig

Clone:	polyclonal

Format:	purified, lyophilized

Quantity:	0.5 ml

Reconstitution:	Reconstitute with 0.5 ml distilled water, store aliquots at -20°C, for further dilution use appropriate antibody diluent e.g. Art. No. PU002

Concentration:	app. 1 mg/ml

Working concentration:	IFA 1:40, IHC(P) 1:200 - 1:600

Presentation:	0.5 ml lyophilized IgG-fraction from ionen exchange chromatography

Immunogen:	Purified fibronectin from chicken plasma

Specificity:	Chicken fibronectin 100%, chicken collagen type I, III and V <0.1% (RIA at 1:10000 dilution)

Species Specificity:	Chicken

General Information:	Fibronectins are glycoproteins, which occur as dimers of two 200 kDa subunits. They have binding domains for bacterial proteins, collagens, heparin-like molecules and fibrin. Cellular fibronectin is widely distributed in the stroma of malignant tumours.

Special properties:	This antibody may be used to identify fibronectin during wound healing, during tumour progression, and tumour invasion respectively.

Positive control:	Chicken skin

Pre-treatment:	After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg e.g. Art. No. HYA02-50) in TBS 15 min at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.

Applications:	IFA, IHC(P), ELISA, RIA

Incubation time:	IHC(P) 60 min at RT or 2-8°C over night

Secondary reagents:	Anti-rabbit IgG-conjugates, e.g. anti-rabbit IgG:Biotin (Art. No. BA-1000)

Storage:	2 - 8°C or aliquots at -20°C.

References:
See list of references in BIOLOGO ECM brochure.

Notes:
BIOLOGO antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.