ECM - FI100: Fibronectin

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FI100: Fibronectin

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Price: 180 € / 1 ml

Monoclonal Antibodies

Origin:	Mouse

Isotype:	IgG1, k

Clone:	568

Format:	liquid conc.

Quantity:	1 ml

Working concentration:	1:10 - 1:50

Presentation:	Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art .No. PU002.

Immunogen:	Purified human Fibronectin from cultivated fibroblasts

Specificity:	Human fibronectin

Species Specificity:	Human

General Information:	Fibronectins are glycoproteins, which occur as dimers of two 200 kDa subunits. They have binding domains for bacterial proteins, collagens, heparin-like molecules and fibrin. Cellular fibronectin is widely distributed in the stroma of malignant tumours.

Special properties:	This antibody may be used to identify fibronectin during wound healing, during tumour progression, and tumour invasion respectively.

Positive control:	Kidney, skin, liver

Pre-treatment:	No protease pre-treatment required. To improve specificity the following procedure may be applied: After de-waxing tissue slices are treated with 0.2% hyaluronidase (app. 300 U/mg e.g. Art. No. HYA02-50) in TBS for 15 min at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.

Applications:	IHC(P)

Incubation time:	60 min at RT

Secondary reagents:	We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6102) or ABC AP (Art. No. AK-5002) systems are applicable.

Storage:	2-8°C

References:
1. Christensen L., et al. (1992) AMPIS Suppl. 26; pp 1-39.

2. Ayard S. Boot-Handfford R.P., Humphries M.J., Kadler K.E., and Shuttleworth C.A. (1994) in The Extracellular Matix Facts Book, Academic Press, London pp. 104-107.

3. Christensen L. (1990) Fibronectin: A discrimination marker between small invasive carcinomas and benign proliferative lesions of the breast. AMPIS 98; 615-623.

Notes:
*BIOLOGO's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.

** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!