ECM - EL25011: Elastin, human

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EL25011: Elastin, human

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Price: 360 € / 0.5 ml

Polyclonal Antibodies

Origin:	Rabbit

Isotype:	Ig

Clone:	polyclonal

Format:	purified, lyophilized

Quantity:	0.5 ml

Reconstitution:	Reconstitute with 0.5 ml distilled water, store aliquots at -20°C, for further dilution use appropriate antibody diluent e.g. Art. No. PU002

Concentration:	app. 1 mg/ml

Working concentration:	IFA 1:80, IHC(P) 1:500, ELISA >1:200

Presentation:	0.5 ml affinity purified antibody lyophilized from phosphate buffered solution; no BSA and preservative added

Immunogen:	Purified elastin from human skin

Specificity:	Human elastin, cross-reactivity in RIA at 1:500 dilution: human collagen type I, II, III, IV, V <0.1 %; human fibronectin < 0.1%

Species Specificity:	Human, cross-reaction with rat < 0.1%

General Information:	Elastin is the major protein of elastic fibres that form a randomly orientated network in many tissues. Due to its high content of hydrophobic amino acids it is relatively resistant to chemicals and proteinase. Its principal function is to provide elasticity to tissues, however it has also chemotactic and cell adhesion properties.

Positive control:	Human skin or aorta

Pre-treatment:	After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg e.g. Art. No. HYA02-50) in TBS 15 min at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.

Applications:	IFA, IHC(P), ELISA, RIA

Incubation time:	IHC(P) 60 min at RT or 2-8°C over night

Secondary reagents:	Anti-rabbit IgG-conjugates, e.g. anti-rabbit IgG:Biotin (Art. No. BA-1000)

Storage:	2 - 8°C or aliquots at -20°C.

References:
See list of references in BIOLOGO ECM brochure.

Notes:
BIOLOGO antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.