ECM - COL007: Collagen Type VII, human

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COL007: Collagen Type VII, human

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Price: 300 € / 1 ml

Monoclonal Antibodies

Origin:	Mouse

Isotype:	IgG1

Clone:	LH7.2

Format:	liquid conc.

Quantity:	1 ml

Concentration:	50 µg/ml

Working concentration:	1:10-1:20

Presentation:	Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art .No. PU002.

Immunogen:	Extract of human neonatal foreskin

Specificity:	Collagen type VII, carboxyterminal

Species Specificity:	Human, Marmoset, Monkey, Cow, Sheep, Goat, and Guinea pig

General Information:	Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists in a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Type VII Collagen consists of alpha 1(VII)-trimeres, which form anchored fibrils . MW alpha-chain app. 170 kDa. Localization: Amnion membrane, basal membrane of skin and oesophagus.

Special properties:	LH7.2 reacts with the basement membrane zone of stratified squamous epithelia. It is useful in differentiating invasive from non-invasive melanoma through clear visualization of appearance and integrity of epidermal basement membrane.

Positive control:	Normal skin

Pre-treatment:	Fixation with ice cold Acetone only (Methanol is not suitable!) No method known for paraffin sections!

Applications:	IHC(C)

Incubation time:	60 min at RT

Secondary reagents:	We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6102) or ABC AP (Art. No. AK-5002) systems are applicable.

Storage:	2-8°C

References:
1. Hegerty A.M.H., et al. (1986) Identification of an epidermal basement membrane defect in recessive forms of dystrophic epidermolysis bullosa by LH7.2 monoclonal antibody: use in diagnosis. Br. J. Dermatol. 115; 125-131.

2. Leigh I.M., et al. (1987) LH7.1 monoclonal antibody detects type VII collagen in basement membrane of ectodermally derived epithelia including skin. Epithelia 1; 17-29.

3. Kirkham N., et al. (1989) Type VII collagen antibody, LH7.2 identifies basement membrane characteristics of thin malignant melanomas. J. Pathol. 157; 243-247.

Notes:
*BIOLOGO's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.

** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!