ECM - CO60411: Collagen Type IV, human

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CO60411: Collagen Type IV, human

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Price: 285 € / 0.1 mg

Monoclonal Antibodies

Origin:	Mouse

Isotype:	IgG1

Clone:	24D1

Format:	purified, lyophilized

Quantity:	0.1 mg

Reconstitution:	Reconstitute by adding 0.1 ml distilled water, for further dilution use appropriate antibody diluent e.g. Art. No. PU002

Concentration:	app. 1 mg/ml

Working concentration:	IFA dilute >1:320 on frozen human skin or liver IHC(P) > 1:4000 tested with polymer kit ELISA >1:4000 will give OD >500

Presentation:	Affinity purified antibody lyophilised from phosphate buffered solution; no BSA and preservative added!

Immunogen:	Purified collagen type IV from human placenta

Specificity:	Human collagen type IV 100% human collagen types I, II, III, and V <0.1% human fibronectin and laminin < 0.1%

Species Specificity:	Human, no cross-reactivity with bovine, mouse, rat and porcine collagens type IV

General Information:	Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs.

Special properties:	Type IV Collagen (170-180 kDa), is a non-fibrillary network of different alpha-chains. It is typically found in basal membranes of different organs (e.g. skin, lens, lung, kidney).

Positive control:	Human skin, placenta or lens capsule

Pre-treatment:	After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg e.g. Art. No. HYA02-50) in TBS 15 min at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.

Applications:	IFA, IHC(P), ELISA, SP-RIA, IB/WB

Incubation time:	IHC(P) 60 min at RT or 2-8°C over night

Secondary reagents:	Anti-mouse IgG-conjugates, e.g. anti-mouse IgG:biotin (Art. No. BA-2000)

Storage:	2 - 8°C or aliquots at -20°C.

References:
1. Guerret G., Govignon E., Hartmann D.J. Ronfard V. (2003) Long term remodeling of a bilayered living human skin equivalent (Apligraf®) grafted onto nude mice: immunolocalization of human cells and characterization of extracellular matrix. Wound Rep. Reg. 11; 35-45.

Notes:
*BIOLOGO's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.