ECM - CO20491: Collagen Type IV, porcine

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CO20491: Collagen Type IV, porcine

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Price: 470 € / 0.5 ml

Polyclonal Antibodies

Origin:	Rabbit

Isotype:	IgG

Clone:	polyclonal

Format:	purified, lyophilized

Quantity:	0.5 ml

Concentration:	app. 1 mg/ml

Working concentration:	IFA 1:80, IHC(P) 1:500, ELISA >1:200

Presentation:	Affinity purified antibody lyophilized from phosphate buffered solution; no BSA and preservative added!

Immunogen:	Purified collagen type IV from porcine kidney tissue

Specificity:	Porcine collagen type IV 100%, cross-reactivity with porcine collagens type I, II, and III <0.1%; porcine elastin, fibronectin and laminin < 0.1

Species Specificity:	Pig, cross-reactivity with human < 0.1, mouse and rat collagen IV <1%

General Information:	Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs.

Special properties:	Type IV Collagen (170-180 kDa), is a non-fibrillary network of different alpha-chains. It is typically found in basal membranes of different organs (e.g. skin, lens, lung, kidney).

Positive control:	Porcine skin or liver

Pre-treatment:	After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg e.g. Art. No. HYA02-50) in TBS 15 min at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.

Applications:	IFA, IHC(P), ELISA, RIA

Incubation time:	IHC(P) 60 min at RT or 2-8°C over night

Secondary reagents:	Anti-rabbit IgG-conjugates, e.g. anti-rabbit IgG:Biotin (Art. No. BA-1000)

Storage:	2 - 8°C or aliquots at -20°C.

References:
See list of references in BIOLOGO ECM brochure.

Notes:
*BIOLOGO's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.