ECM - CO20441: Collagen type IV, rat

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CO20441: Collagen type IV, rat

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Price: 425 € / 0.5 ml

Polyclonal Antibodies

Origin:	Rabbit

Isotype:	Ig

Clone:	polyclonal

Format:	purified, lyophilized

Quantity:	0.5 ml

Reconstitution:	Reconstitute with 0.5 ml distilled water, store aliquots at -20°C, for further dilution use appropriate antibody diluent e.g. Art. No. PU002

Concentration:	app. 1 mg/ml

Working concentration:	IFA 1:80, IHC(P) 1:200 - 1:600, ELISA 1:200 gives OD>500

Presentation:	0.5 ml affinity purified antibody lyophilized from phosphate buffered solution; no BSA and preservative added

Immunogen:	Purified collagen type IV from ruminant tumour tissue

Specificity:	Rat collagen type IV 100%; rat collagen type I, II, III and V <0.1%; human collagen type IV <0.1%; rat and murine fibronectin <0.1%; murine laminin <0.1% in RIA at 1:5000 dilution

Species Specificity:	Rat

General Information:	Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs.

Special properties:	Type IV Collagen (170-180 kDa), is a non-fibrilary network of different alpha-chains. It is typically found in basal membranes of different organs (e.g. skin, lens, lung, renals).

Positive control:	Rat skin or liver

Pre-treatment:	After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg e.g. Art. No. HYA02-50) in TBS 15 min at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.

Applications:	IFA, IHC(P), ELISA, RIA

Incubation time:	IHC(P) 60 min at RT or 2-8°C over night

Secondary reagents:	Anti-rabbit IgG-conjugates, e.g. anti-rabbit IgG:Biotin (Art. No. BA-1000)

Storage:	2 - 8°C or aliquots at -20°C.

References:
See list of references in BIOLOGO ECM brochure.

Notes:
BIOLOGO antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.