ECM - CO20221-0.1: Collagen type II, bovine

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CO20221-0.1: Collagen type II, bovine

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Price: 210 € / 0.1 ml

Polyclonal Antibodies

Origin:	Rabbit

Isotype:	Ig

Clone:	polyclonal

Format:	purified, lyophilized

Quantity:	0.1 ml

Reconstitution:	Reconstitute by adding 0.1 ml distilled water, for further dilution use appropriate antibody diluent e.g. Art. No. PU002

Concentration:	app. 1 mg/ml

Working concentration:	IFA 1:40, IHC(P) 1:100 - 1:500

Presentation:	0.5 ml affinity purified lyophilised IgG-fraction, no preservative or BSA added!

Immunogen:	Purified collagen type II from bovine cartilage

Specificity:	Bovine collagen type II 100%, bovine collagen XI <3.0 %; bovine collagen I and IX <0.1% (RIA at 1:200 dilution)

Species Specificity:	Cattle, cross-reactivity with horse quite strong, low reactivity with other species like rat, pig etc.

General Information:	Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs.

Special properties:	Type II collagen is an alpha1(II)-trimer, MW 95 kDa, which forms 67 nm cross-banded fibrils. Typically it can be observed in skin, cartilage and various tumours.

Positive control:	Bovine cartilage

Pre-treatment:	After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg e.g. Art. No. HYA02-50) in TBS 15 min at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.

Applications:	IFA, IHC(P), ELISA, RIA

Incubation time:	IHC(P) 60 min at RT or 2-8°C over night

Secondary reagents:	Anti-rabbit IgG-conjugates, e.g. anti-rabbit IgG:Biotin (Art. No. BA-1000)

Storage:	2 - 8°C or aliquots at -20°C.

References:
See list of references in BIOLOGO ECM brochure.

Notes:
BIOLOGO antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.