BioPrime Antibodies - CK520: Cytokeratin 20

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CK520: Cytokeratin 20

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Price: 175 € / 5 ml

Monoclonal Antibodies

Origin:	Mouse

Isotype:	IgG1

Clone:	Ks20.8

Format:	ready to use

Quantity:	5 ml

Working concentration:	neat

Presentation:	Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 50 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art. No. PU002, if further dilution is required.

Immunogen:	Human duodenal microvilli

Specificity:	Acid Cytokeratin 20, 46 kD

Species Specificity:	Human

General Information:	Cytokeratins are a group of water-insoluble filamentous proteins. They are constituents of the cytoskeleton of epidermal cells and other epithelial cells. Up to now 20 different cytokeratins have been characterised by gel electrophoresis. They have been subdivided into a basic and an acid subfamily. Most frequently the nomenclature according to Moll et al. (1982) is used.

Special properties:	Cytokeratin 20 is an acid cytokeratin of low MW (46 kDa), which is exprimed by gastrointestinal epithelia, urothel, Merkel-cells of the epidermis as well as by some endocrine cells in the pylorus. Most other epithelia including breast don't exprime CK20. Colorectal carcinoma frequently exprime CK20, while adenocarcinoma of stomach gall bladder, gall ducts, pancreas, mucinous ovarian cancer and transitional cell tumours exprime this CK to a minor extent. Negative staining is usually obtained in adenocarcinoma of breast, lung, endometrium and non-mucinous tumours of the ovary. For differential diagnosis of ovarian carcinoma and colorectal carcinoma CK20 antibody should be used in combination with CK7 (Art. No. CK107 and CK507) only.

Positive control:	Appendix, colon carcinoma

Pre-treatment:	Heat pre-treatment of formalin-fixed tissue with Unmasking Fluid G (Art. No. DE007)

Applications:	C, P

Incubation time:	60 min at RT

Secondary reagents:	We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6102) or ABC AP (Art. No. AK-5002) systems are applicable.

Storage:	2-8°C

References:
1. Moll R., Franke W.W., Schiller D.L., Geiger B., and Krepler R. (1982) The Catalog of Human Cytokeratins: Patterns of Expression in Normal Epithelia, Tumors and Cultured Cells. Cell 31; 11 ff.

2. Sun T.-T. Tseng S.C.G., Huang A.J.W., Cooper D., Lynch M.H., Weiss R., Eichner R., and Schermer (1985) Monoclonal antibody studies of keratin expression: A review. In: Intermediate Filaments , Wang E. et al. eds. N.Y. Acad. Sci. 455, pp 307 ff.

3. Moll R., L

4. Wauters CC., Smedts, F., Gerrits L.G., Bosman, F.T. , and Ramaekers F.C. (1995) Keratins 7 and 20 as diagnostic markers for carcinomas metastatic to the ovary. Hum. Pathol. 26(8); 852-855.

5. De La Rosette J., Smedts F., Schoots C., Hoek H., and P. Laguna (2002) Changing patterns of keratin expression could be associated with functional maturation of the developing human bladder. J. Urol. 168(2); 709-717.

Notes:
*BIOLOGO's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.

** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!