BioPrime Antibodies - CD45RO-5: CD45RO, T-cell subset, human

Products:

AGE

BioPrime Antibodies

BioPrime Staining Reagents

Proteins and Peptides

VECTOR Reagents

Products> BioPrime Antibodies

CD45RO-5: CD45RO, T-cell subset, human

Print page

place in shopping cart

Price: 150 € / 5 ml

Monoclonal Antibodies

Origin:	Mouse

Isotype:	IgG2a

Clone:	UCHL-1

Format:	ready to use

Quantity:	5 ml

Working concentration:	neat

Presentation:	Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 50 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art. No. PU002, if further dilution is required.

Immunogen:	T-cell line CA1

Specificity:	Human CD45RO membrane glycoprotein, 180 kDa on memory T-cells

Species Specificity:	Human

General Information:	The group of CD45 membrane glycoproteins consists of 5 different isoforms, which represent different functional status of the T-lymphocytes. CD45RO is mainly found on thymocytes and a subpopulation of CD4+ and CD8+ T-cells (Memory T-cells). Resting T-cells mainly bear CD45RA molecules on the surface, which get lost after activation by allo-antigens, and is substituted by CD45RO. Normal B-cells and NK-cells are CD45RO negative.

Special properties:	The antibody may be used to assess the status of activity of T-cell populations. It is meaningful to compare with the performance of antibodies to CD45 (Art. No. LC500) or CD3 (Art. No. TC500).

Positive control:	Lymph node, tonsil

Pre-treatment:	Pre-treatment with unmasking fluids E (Art. No. DE006) or C (Art. No. DE000).

Applications:	IHC(P)

Incubation time:	60 min at RT

Secondary reagents:	We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6102) or ABC AP (Art. No. AK-5002) systems are applicable.

Storage:	2-8°C

References:
1. Smith S.H., Brown M.H., Rowe D., Callard R.E., Beverley P.C.L. (1986) Functional subsets of human helper-inducer cells defined by a new monoclonal antibody, UCHL1. Immunology 58; 63-70.

2. Terry L.A., Brown M.H., Beverley P.C.L. (1988) The monoclonal antibody UCHL1 recognizes a 1800 MW component of the human leucocyte common antigen, CD45. Immunology 64; 331-336.

3. Akbar A.N., Terry L.A., Timms A., Beverley P.C.L., Janossy G. (1988) Loss of CD45R and gain of UCHL1 reactivity is a feature of primed T cells. J. Immunol. 140; 2171-2178.

4. Morimoto C. (1995) CD45 Cluster Report, In: Schlossman S.F. et al. Eds. Leucocyte Typing V. White cell differentiation antigens. Oxford University Press, Oxford, N.Y., Tokyo; pp 386-391.

Notes:
*BIOLOGO's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.

** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!