BioPrime Antibodies - CA199-1: CA 19-9, Sialyl-Lewis a

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CA199-1: CA 19-9, Sialyl-Lewis a

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Price: 330 € / 1 ml

Monoclonal Antibodies

Origin:	Mouse

Isotype:	IgM

Clone:	121 SLE

Format:	liquid conc.

Quantity:	1 ml

Concentration:	0.1 mg/ml

Working concentration:	1:10 - 1:50

Presentation:	Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art .No. PU002.

Immunogen:	Immunodiffusion precipitate of ovarian cystic mucins with NS19-9-antibody

Specificity:	Sialyl Lewis a antigen (Cancer Antigen CA 19-9)

Species Specificity:	Human

General Information:	Sialyl Lewis a antigen is an oncofetal antigen, which appears during early development of the gastrointestinal tract. After birth it is still detectable in ductus and secretion of intestinal glands, respiratory and reproductive tract. In normal tissue Sialyl Lewis a (CA 19-9) is found in ductal epithelia of the breast, kidney, salivary glands, lung, colon, bile duct, liver, pancreas and prostate gland.

Special properties:	CA 19-9 antibody reacts strongly positive with most tumour cell lines of gastrointestinal carcinoma including adenocarcinoma of the stomach, intestine, and pancreas. Positive reactivity was also found in transitional cell carcinoma of the bladder, adenocarcinoma of the endometrium and the gallbladder as well as in papillary carcinoma of the thyroid glands. Last but not least positive reactivity of lung carcinoma must be mentioned (adenocarcinoma, bronchio-alveolar carcinoma, squamous epithelial carcinoma, and SCLC).

Positive control:	Colon carcinoma

Pre-treatment:	In paraffin section no pre-treatment is required, however heat induced antigen retrieval may improve the staining. Each laboratory should select it's own optimum staining and pre-treatment conditions.

Applications:	ELISA, IRMA, IHC (cryostat and paraffin sections)

Incubation time:	60 min at RT

Secondary reagents:	We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6102) or ABC AP (Art. No. AK-5002) systems are applicable.

Storage:	2-8°C

References:
1. Gatalica Z., and Miettienen M. (1994) Distribution of carcinoma antigens CA 19-9 and CA 15-3: an immunohistochemical study of 400 tumors. Appl. Immunohistochem. 2(3); 205ff.

2. Tomazic A., and Pegan V. (2000) Preoperative staging of periampullar cancer with US, CT, EUS and CA 19-9. Hepatogastroenterol. 47(34); 1135-1137.

3. Tabata T., Takeshima N., Tanaka N., Hirai Y., and Hasumi K. (2000) Clinical value of tumor markers for early detection of recurrence in patients with cervical adenocarcinoma and adenosquamous carcinoma. Tumour Biol. 21(6); 375-380.

4. Dalgleish A.G. (2000) Tumour markers in malignancies. CA19.9 is useful in several cancers. B.M.J. 321(7257); 380

Notes:
*BIOLOGO's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.

** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!