AGE - CML011: CML, N-Epsilon-(Carboxymethyl)-Lysine

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CML011: CML, N-Epsilon-(Carboxymethyl)-Lysine

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Price: 670 € / 50 µg

Monoclonal Antibodies

Origin:	Mouse

Isotype:	IgG1

Clone:	CMS-10

Format:	liqiud

Quantity:	50 µg

Concentration:	0.25 mg/ml

Working concentration:	ELISA 0.1-0.5 µg/ml

Presentation:	Protein G affinity purified antibody from ascites in stabilized buffer, containing 50% Block Ace? (Casein-containing solution, Dainippon Co.) and 0.1% ProClin? (Rohm & Haas) as a preservative

Immunogen:	N-epsilon-(Carboxymethyl)-Lysin (CML) konjugated to KLH

Specificity:	Reaction with CML-HSA, CML-BSA, and free CML in competitive ELISA, no cross-reactivity with CEL, MG-derived AGE, but with Glycolaldehyde-AGE and Glyoxal-AGE (Koito et al., 2004)

General Information:	Long-term incubation of proteins with glucose leads, through Schiff's base and Amadori rearrangement products, to the formation of advanced glycation end products (AGE) which are characterized by fluorescence, brown color and inter- and intra-molecular cross-linking. Recent immunological studies using anti-AGE antibodies demonstrated the presence of AGE in (i) human lens, (ii) renal proximal tubules in patients with diabetic nephropathy and chronic renal failure, (iii) atherosclerotic lesions of arterial walls, (iv) ß2-microglobulin of carpal tunnel amyloid fibril deposits in patients with hemodialysis-related amyloidosis and (v) brain tissues of patients with Alzheimer?s disease. N-epsilon-(carboxymethyl)-lysine was identified to be a major structure in AGE (Dunn et al., 1989). Oxidative cleavage of Amadori-products seems to be the main pathway of CML-formation in vivo. More recent investigations however have shown, that as well lipid-peroxidation as glycoxidation can be involved in CML-build up in vivo (Fu et al., 1996).

Special properties:	This antibody is suitable for the detection of CML in tissues and tissue extracts.

Applications:	ELISA, (IHC not estabished)

Incubation time:	60 min at RT

Secondary reagents:	We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC Mouse IgG(Art. No. PK-6102) or ABC AP Mouse IgG (Art. No. AK-5002) systems are preferable.

Storage:	-20°C

References:
1. Horiuchi S., Araki N., and Morino Y. (1991) Immunological approach to characterize advanced glycation end products of the Maillard reaction: Evidence for the presence of a common stucture. J. Biol. Chem. 266; 7329-7332.

2. Dunn J.A., Patrick J.S., Thorpe S.R., Baynes J.W. (1989): Oxidation of glycated proteins: age-dependent accumulation of N-epsilon-(carboxymethyl) lysine in lens proteins. Biochemistry. 28: 9464-9468.

3. Fu M.X., Requena J.R., Jenkins A.J., Lions T.J., Baynes J.W., Thorpe S.R. (1996): The advanced glycation end product, N-epsilon-(carboxymethyl) lysine, is a product of both lipid peroxidation and glycoxidation reactions. J.Biol.Chem.271: 9982-9986

Notes:
This antibody is intended for in vitro research use only. It must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.