AGE - AGE612: AGE, Advanced Glycation End Products

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AGE612: AGE, Advanced Glycation End Products

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Price: 670 € / 10 µg

Monoclonal Antibodies

Origin:	Mouse

Isotype:	IgG1

Clone:	6D12

Format:	liqiud

Quantity:	10 µg

Concentration:	0.25 mg/ml

Working concentration:	ELISA 0.1-0.5 µg/ml; IHC 2 µg/ml

Presentation:	Protein G affinity purified antibody from ascites in stabilized buffer, containing 50% Block Ace? (Casein-containing solution, Dainippon Co.) and 0.1% ProClin? (Rohm & Haas) as a preservative

Immunogen:	Advanced Glycation End Products-BSA

Specificity:	Positive: Advanced Glycation Endproducts, AGE-HSA, AGE-BSA, AGE-Hb, AGE-Collagen,AGE-Lys-derivatives (AGE-alpha-Tos-Lys, AGE-alpha-Tos-Lys-o-Me), AGE-monoamino carboxylic acids (AGE-beta-Alanine, AGE-gamma-aminobutyric acid, AGE-epsilon-aminocaproic acid) - negativ: early stages of the Maillard reaction (Schiff's base adducts, Amadori rearrangement products), unmodified protein HSA, BSA, Hb, Collagen, poly-Lys, Lys-derivatives (alpha-Tos-Lys, alpha-Tos-Lys-o-Me), unmodified monoamino carboxylic acids (beta-alanine, gamma-amino butyric acid, epsilon-aminocaproic acid), FFI, Pyrrole aldehyde, Pentosidine.

General Information:	Long-term incubation of proteins with glucose leads, through Schiff's base and Amadori rearrangement products, to the formation of advanced glycation end products (AGE) which are characterized by fluorescence, brown color and inter- and intra-molecular cross-linking. Recent immunological studies using anti-AGE antibodies demonstrated the presence of AGE in (i) human lens, (ii) renal proximal tubules in patients with diabetic nephropathy and chronic renal failure, (iii) atherosclerotic lesions of arterial walls, (iv) ß2-microglobulin of carpal tunnel amyloid fibril deposits in patients with hemodialysis-related amyloidosis and (v) brain tissues of patients with Alzheimer?s disease. These results suggested the potential role of AGE in normal aging and age-enhanced disease processes.

Special properties:	This antibody is suitable for the detection of different AGE products in tissues, tissue extracts and body fluids.

Positive control:	human lens, arteriosclerotic plaques

Applications:	IHC, ELSA

Incubation time:	60 min at RT

Secondary reagents:	We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC Mouse IgG(Art. No. PK-6102) or ABC AP Mouse IgG (Art. No. AK-5002) systems are preferable.

Storage:	-20°C

References:
1. Horiuchi S., Araki N., and Morino Y. (1991) Immunological approach to characterize advanced glycation end products of the Maillard reaction: Evidence for the presence of a common stucture. J. Biol. Chem. 266; 7329-7332.

2. Akari N., Ueno N., Chakrabarti B., Morino Y., and Horiuchi S. (1992) Immunochemical evidence of the presence of advanced glycation end products in human lens proteins and its positive correlation with aging. J. Biol. Chem. 267; 10211-10214.

3. Miyata T., Odo O., Inagi R., Iida Y., Araki N., Yamada N., Horiuchi S., Taniguchi N., Maeda, et al. (1993) ß2-Microglobulin modified with advanced glycation end products is a major component of haemodialysis-associated amyloidosis. J. Clin. Invest. 92; 1243-1252.

4. Kume S., Takeya M., Mori T., Araki N., Suzuki H., Horiuchi S., Kodama T., Miyauchi Y., and Takahashi K. (1995) Immunohistochemical and ultrastructural detection of advanced glycation end products in atherosclerotic lesions of human aorta using a novel specific monoclonal antibody. Am J. Pathol. 147; 654-667.

5. Kimura T., Takamatsu J., Ikeda K., Kondo A., Miyakawa T., and Horiuchi S. (1996) Accumulation of advanced glycation end products of the Maillard reaction with age in human hippocampal neurons. Neurosci. Lett. 208; 53-56.

Notes:
This antibody is intended for in vitro research use only. It must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.