AGE - AGE06B: Methylglyoxal-AGE (Arg-Pyrimidine)

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AGE06B: Methylglyoxal-AGE (Arg-Pyrimidine)

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Price: 670 € / 30 µg

Monoclonal Antibodies

Origin:	Mouse

Isotype:	IgG2a

Clone:	6B

Format:	liquid

Quantity:	30 µg

Concentration:	100 µg/ml

Working concentration:	1 µg/ml

Presentation:	Antibody purified by ammonium sulphate from ascites, taken up in 10 mM PBS; 0.5% BSA; 0.1% NaN3

Immunogen:	Methylglyoxal-KLH-adduct

Specificity:	Methylglyoxal (MG)-Adducts, primarily Arg-Pyrimidine (N-delta-(4-Carboxy-4,6-Dimethyl-5,6-Dihydroxy-1,4,5,6-Tetrahydropyrimidine-2-yl)-L-Ornithine), cross-reactivity with Tetrahydropyrimidine and 5-Hydro-5-Methylimidazolone app. 0.1% in ELISA, Immidazolysine and (Carboxyethyl)lysine <0,1%

General Information:	Methylglyoxal (MG) is a cytotoxic metabolite, which is synthesized as a side product of glycolysis. In diabetic hyperglycemia accumulation of MG and irreversible binding to cellular proteins especially Arg- and Lys-residues may occur. This leads to the formation of "Advanced Glycation End Products" (AGE). The formation of MG-derived AGE contributes to several pathological conditions including artherosclerosis, Alzheimer's disease and complications in diabetes (blindness, neuropathy, nephropathy). The glyoxalase-1-system (GLO1) is stimulated in different tumours (Colon-, Mamma- and Prostate-Ca) and correlates with the very fast formation of MG-adducts in TNF-stimulated Apoptosis. This leads to the conclusion that in those cases AGE products are formed through enzymatic reactions.

Special properties:	The antibody 6B is suitable for the detection of MG-derived AGE especially of Arg-Pyrimidine in tissues and tissue extracts. Special focus will be put on the investigation of arterial sclerosis and protein deposits in the brain, renals, retina and lens.

Positive control:	Nephropathic renal tissue

Applications:	C, P, WB

Incubation time:	60 min at RT

Secondary reagents:	We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC Mouse IgG(Art. No. PK-6102) or ABC AP Mouse IgG (Art. No. AK-5002) systems are preferable.

Storage:	-20°C

References:
1. Oya T., Hattori N., Mizuno Y., Miyata S., Maeda S., Osawa T., and K. Uchida (1999) Methylglyoxal modification of protein. Chemical and immunochemical characterization of methylglyoxal-arginine adducts. J. Biol. Chem. 274(26); 18492-18502.

2. Van Herreweghe F., Mao J., Chaplen F.W.R., Grooten J., Gevaert K., Vandekerckhove J., and K. Vancompernolle (2002) Tumor necrosis factor-induced modulation of glyoxalase 1 acticvities through phosphorylation by PKA results in cell death and is accompanied by the formation of a specific methylglyoxal-derived AGE. PNAS 99(2); 949-954.

3. Shipanova I.N., Glomb M.A., and R.H. Nagaraj (1997) Protein Modification by Methylglyoxal: Chemical nature and synthetic mechanism of a major fluorescent adduct. Arch. Biochem. Biophys.344(1); 29-36.

4. Padayatti P.S., Ng A.S., Uchida K., Glomb M.A., and R.H. Nagaraj (2001) Argpyrimidine, a blue fluorophore in human lense proteins: high levels in brunescent cataractous lenses. Invest Ophthalmol. Vis. Sci. 42(6); 1299-1304.

5. Padayatti P.S., Jiang C., Glomb M.A., Uchida K., and R.H. Nagaraj (2001) High concentrations of glucose induce synthesis of argpyrimidine in retinal endothelial cells. Curr. Eye Res. 23(2); 106-115.

Notes:
** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

BIOLOGO reagents are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.